瀚海新酶提供载体构建、瞬时转染表达、稳转表达及蛋白纯化等一站式服务。

Mammalian Cell Protein Expression System Service

Mammalian Cell Protein Expression System Service

Mammalian cells possess complex protein folds and Post-translational modification function, which means it can express recombinant proteins more close to natural proteins in terms of in molecular structure, physicochemical properties and biological functions. Therefore, it is more likely to obtain proteins with the same biological activity, which is extremely important in the production of recombinant proteins especially therapeutic antibodies. Hzymes Biotech provides one-stop CRO services including plasmid construction, transient transfection expression, stable expression, and protein purification.

Equipped with mature development routes from codon optimization, protein expression, purification, and protein trait analysis

Possess a variety of expression system for choice and for successful expression recombinant proteins or antibodies

Abandunt experimental equipment, good experimental environment and professional team to ensure the stability and rapid delivery of service

· Workflow ·

- DELIVERY STANDARDS -

Service content	Delivery content	Period
Sequence optimization and vector construction	Sequence information and expression plasmids	2-3Weeks
Transient expression	Experimental report, protein sample	2-4Weeks
Stable expression (optional)	Stable cell line (Top5), construction report	2-4Weeks
Small scale shaking bottle and protein purification	Protein samples and expression evaluation reports	3-4Weeks

- CASE -

Case Description:The original expression level of the target secreted protein required by the customer is relatively low. Based on this Hymes platform process, we attempted to replace different signal peptides and optimize the expression level by combining them with specialized carriers. After optimization, the protein expression level has increased by tens of times compared to the original level, and the functionality of the finished product meets the customer's needs.

Case 1: The advantage of platform technology helps significantly increase expression volume